Detection of Albumin in Urine – MeitY OLabs
Detection of Albumin in Urine Albumin is a protein produced by the liver
and is mainly found in the blood. A trace of protein is found in normal urine daily.
Under pathological conditions like Albuminuria, the level of albumin found in urine will be
way above the normal level. In kidney disturbance and in high blood pressure, albumin level
in urine is significantly high. To detect albumin in the urine, Sulphosalicylic acid
test and Heller’s tests are done. Sulphosalicylic Acid Test Materials Required: Urine sample in a test tube, 30% Sulphosalicylic
acid and a dropper. Procedure: Using a dropper, take a small quantity of
sulphosalicylic acid. Now, add it to the test tube containing the
urine sample. A cloudy turbid solution or precipitate appearing
in the solution indicates the presence of albumin in the urine sample. Conclusion: When sulphosalicylic acid reacts with albumin,
it causes denaturation of protein in the sample that appears in the form of precipitate. Heller’s Test Materials required: Concentrated nitric acid in a test tube, urine
sample and a dropper. Procedure: Using a dropper, take a small quantity of
urine from the urine sample bottle. Take the test tube containing concentrated
nitric acid. Incline the test tube and add the sample of
urine using a dropper along the inner side of the test tube.
As the urine comes in contact with the nitric acid, a white ring is formed at the point
of contact. The presence of a white ring at the junction
of two layers indicates the presence of albumin in the sample. Conclusion: Nitric acid causes denaturation of proteins
with the formation of a white precipitate. As urine that contains albumin comes in contact
with the nitric acid, it forms a white ring at the point of contact.